PARP4 Mouse Monoclonal Antibody [Clone ID: P193-10]
CAT#: AM32039SU-N
PARP4 mouse monoclonal antibody, clone P193-10, Liquid
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CNY 6,537.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | P193-10 |
Applications | IHC, WB |
Recommend Dilution | Western blot: 1/10-1/20 (preferably with ECL). Immunohistochemistry: Use 1/10-1/20 for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. Pretreatment: 10 min 20 mM Glycine in PBS (pH 7.5) and 10 min 6 N Guanidine Hydrochloride in 50 mM Tris-HCl, pH 7.5 (See Schroeijers et al., 2000). |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | E. coli lysate transformed with the pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA. |
Specificity | This Monoclonal antobofdy P193-10 reacts with an internal epitope (amino acids 506-510, VALGK) of the minor vault protein (p193 or VPARP), which is overexpressed in various Human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles. |
Formulation | State: Liquid Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Gene Name | poly(ADP-ribose) polymerase family member 4 |
Database Link | |
Background | PARP-1 is a nuclear protein that is specifically cleaved by caspase-3 and caspase-6, but not by caspase-1, into a signature apoptotic fragment. PARP-2 and PARP-3 interact with PARP-1. PARP-4, also designated vault poly(ADP-ribose) polymerase (VPARP) and ADP-ribotransferase-like 1 (ADPRTL1), associates with the major vault protein (MVP) and telomerase-associated protein 1 (TEP1) to form vaults, barrel-shaped cytoplasmic ribonucleoprotein particles. PARP-4 localizes mainly to the cytoplasm but is also found in the nucleus. The PARP-4 protein is expressed widely, with highest levels observed in the kidney, and is also detected in skeletal muscle, heart, leukocytes, placenta, lung, liver, spleen, and pancreas. PARP-4 contains a PARP (ADPRT)-like catalytic domain, a C-terminal MVP-interacting domain, a domain with two sequences similar to inter-alpha-trypsin inhibitor, and an N-terminal BRCA1 C-terminus (BRCT) domain, which may be involved in protein-protein interactions. |
Synonyms | PARP-4, ADPRTL1, KIAA0177, PARPL, VPARP |
Reference Data |
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