MRP5 (ABCC5) Rat Monoclonal Antibody [Clone ID: M5I-10]

Specifications

Product Data
Clone Name	M5I-10
Applications	IF, IHC, WB
Recommend Dilution	Western blotting: Use at 1/20-1/50 and anti-Rat-HRP. Immunocytochemistry: Use at 1/20-1/50 on Acetone Fixed Cytospin preparations. Immunohistochemisty on Frozen Sections: 1/20 on Acetone Fixed Frozen sections can be followed by incubation with Rabbit anti-Rat Ig (1/25) and a monoclonal Rat APAAP complex (1/50). Alternatively, after incubation with M5I-10 (use 1/20) and washing, slides can be incubated with biotinylated Rabbit anti-Rat Ig (1/100) and streptavidin conjugated to horseradish peroxidase (1/500).
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	A bacterial fusion protein of Mouse Mrp5, containing amino acids 1-38 of the protein.
Specificity	This Monoclonal antibody M5I-10 reacts with an internal epitope of MRP5 (ABCC5), an approximately 160 kD transmembrane protein that is related to the multidrug resistance protein MRP1. It also strongly reacts with the Human MRP5 protein.
Formulation	State: Supernatant State: Serum Free Culture Supernatant Stabilizer: 0.7% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store antibody undiluted at 2-8°C.
Gene Name	ATP binding cassette subfamily C member 5
Database Link	Entrez Gene 10057 Human UniProt ID O15440
Background	MRP5 (190-200 kDa) is closely related to MRP4, both lacking the first five membrane spanning regions. MRP5 is a GS-X multi specific organic anion pump (nucleotide analogs). MRP5 may transport DNP-GS and may be inhibited by certain inhibitors of organic anion transport (sulfinpyrazone). MRP5 may also transport organic anions with the anionic moiety of phosphate/phosphonate group, a function which provides the ability to resist against anti cancer drugs 6-MP and thioguanine as well as the anti-HIV drug PMEA.
Synonyms	Multidrug resistance-associated protein 5, pABC11, SMRP, MOAT-C
Reference Data

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