Il10 Mouse Monoclonal Antibody
CAT#: AM31967PU-N
Il10 mouse monoclonal antibody, Aff - Purified
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CNY 3,630.00
货期*
5周
规格
Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 8.0-16.0 µg/ml of this antibody will detect at least 500 pg/well of recombinant Rat IL-10 when used in conjunction with Biotinylated antigen affinity purified anti-Rat IL-10 (Cat.-No PP1115B1/PP1115B2) as the detection antibody at a concentration of at least 0.5-1.0 µg/ml. Western Blot: To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions. |
Reactivity | Rat |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | E.coli derived Recombinant Rat IL-10 (Cat.-No PA041). |
Specificity | This antibody recognizes Interleukin-10 / IL10 |
Formulation | PBS State: Aff - Purified State: Lyophilized purified tissue culture |
Reconstitution Method | Centrifuge the vial prior to opening and restore in water to a concentration of 1.0 mg/ml. |
Purification | Affinity Chromatography Protein A |
Conjugation | Unconjugated |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | interleukin 10 |
Database Link | |
Background | IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells. |
Synonyms | IL-10, CSIF, TGIF |
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