Cd80 Rat Monoclonal Antibody [Clone ID: RMMP-1]

Specifications

Product Data
Clone Name	RMMP-1
Applications	FC
Recommend Dilution	Flow Cytometry (See Protocols).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises the murine CD80 cell surface protein, also known as B7-1.
Formulation	PBS containing 0.09% Sodium Azide as preservative and EIA grade BSA to bring total protein concentration to 4-5 mg/ml State: Purified State: Liquid purified IgG fraction Label: R-Phycoerythrin (R-PE)
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Gene Name	CD80 antigen
Database Link	Entrez Gene 12519 Mouse UniProt ID Q00609
Background	CD80 is a member of the Ig superfamily, along with CD86 (B7-2), participates in T cell costimulation via interactions with CD28 and CTLA-4. CD80 is constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages; and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 is greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours.
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Note	Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 μl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add ~1.0 μg* of AM31878RP-N or AM31878RP-L per 1x10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes at 4°C for 30 minutes. (It is recommended that the tubes are protected from light since most fluorochromes are light sensitive). 7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 μl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 μl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 μl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 μl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: C3H Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 1.0 μg/106 cells Isotypic Control: PE Rat IgG2a
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.