Cd5 Rat Monoclonal Antibody [Clone ID: 53-7.3]

Specifications

Product Data
Clone Name	53-7.3
Applications	FC
Recommend Dilution	This clone (53-7.3) has been reported to work in Immunohistochemistry on Acetone Fixed Frozen Sections and Zinc Fixed Paraffin Sections, Immunoprecipitation and Flow Cytometry.
Reactivity	Human, Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Mouse thymus or spleen from Balb/c mouse thymus or spleen.
Specificity	This CD5 (Ly-1) Monoclonal Antibody is specific for the Mouse CD5 antigen.
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml Label: Biotin State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in alqiuots) -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD5 antigen
Database Link	Entrez Gene 921 HumanEntrez Gene 12507 Mouse UniProt ID P13379
Background	CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.
Synonyms	CD5, LEU1
Note	Protocol: Test Results: Tissue Distribution by Flow Cytometry Analysis: Cell Concentration: 1x10e6 cells per test Antibody Concentration Used: 0.5 μg/10e6 cells Secondary Antibody Used: Streptavidin-FITC 1/500 dilutions. Cell Source Percentage of cell stained above control: Thymus: 98.38% Spleen: 39.19% Bone Marrow: 3.66%
Reference Data

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