S100A9 Mouse Monoclonal Antibody [Clone ID: CF-557]

Specifications

Product Data
Clone Name	CF-557
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 2.0 µg/106 cells. Positive Control: Human peripheral blood leukocytes. Immunofluorescence (Reported). Western blot (Reported). Immunohistochemistry (Reported).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified granulocyte antigen of Human origin.
Specificity	This antibody Binds to Human Calgranulin B (S100A9). Other species not tested.
Formulation	PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. Label: PE State: Liquid purified IgG fraction from Ascitic fluid
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	PE
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	S100 calcium binding protein A9
Database Link	Entrez Gene 6280 Human UniProt ID P06702
Background	Calgranulin B, also known as MRP-14 and S100A9 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. MRP-14 (S100A9) forms a heterodimeric complex with MRP-8 (S100A8) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin B is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma. Calgranulin B is also expressed on macrophages in acute inflamed tissues (peridontitis, contact excema).
Synonyms	S100-A9, CAGB, MRP-14
Note	Protocol: Tests Results Tissue Distribution by Flow Cytometry Analysis: Cell Concentration: 1x106 cells per tests. Antibody Concentration Used: 2.0 μg/106 cells. Isotypic Control (shaded): Purified Mouse IgG1.
Reference Data

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