MRP8 (S100A8) Mouse Monoclonal Antibody [Clone ID: CF-145]

Specifications

Product Data
Clone Name	CF-145
Applications	FC, IF, IHC, WB
Recommend Dilution	Flow Cytometry: 0.5 µg/1x106 cells. Immunofluorescence. Western blot. Immunohistochemistry on Paraffin Sections: 1/50. Heat induced antigen retrieval in pH 6.0 citrate buffer is recommended.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified granulocyte antigen of Human origin. Donor: Human leukocytes. Fusion Partner: NSO Mouse Myeloma Partner.
Specificity	This antibody detects Calgranulin A (S100A8).
Formulation	PBS Label: FITC State: Liquid purified IgG fraction of Ascitic fluid Stabilizer: EIA grade BSA to bring total protein concentration to 4-5 mg/ml Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	S100 calcium binding protein A8
Database Link	Entrez Gene 6279 Human UniProt ID P05109
Background	Calgranulin A, also known as MRP-8 and S100A8 is a member of the S100 family of proteins containing 2 EF hand (alpha helix, turn, alpha helix structure) calcium binding motifs. S100 proteins are localized in the cytoplasm and /or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. MRP-8 (S100A8) forms a heterodimeric complex with Calgranulin B, (MRP-14, S100A9) in the cytosol of monocyte and neutrophil cell types circulating in peripheral blood. Calgranulin A is found in elevated levels in the serum of cystic fibrosis cases and is also expressed in the skin of patients with psoriasis, eczematous dermatitis and squamous cell carcinoma.
Synonyms	S100-A8, CAGA, MRP-8, CFAG
Note	Protocol: Tests Results Tissue Distribution by Flow Cytometry Analysis: Cell Concentration : 1x10e6 cells per tests. Antibody Concentration Used: 0.5 μg/10e6 cells. Isotypic Control (shaded): FITC Mouse IgG2b.
Reference Data

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