VEGF Receptor 2 (KDR) (3rd Extracell. Dom.) Rat Monoclonal Antibody [Clone ID: BEP-5D8]

Specifications

Product Data
Clone Name	BEP-5D8
Applications	ELISA, FC, WB
Recommend Dilution	Flow Cytometry: 1.2 μg/106 cells. ELISA: 1/200-1/400. Cell based ELISA: 1/200-1/400.
Reactivity	Human, Porcine
Host	Rat
Clonality	Monoclonal
Immunogen	genetic immunisation with cDNA encoding Human VEGFR2. Selection: based on recognition of the complete native protein expressed on transfected mammalian cells.
Specificity	This antibody detects VEGFR2 (epitope on the third extracellular immunoglobulin-like domain).
Formulation	Phosphate buffered saline, pH 7.2 State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	kinase insert domain receptor
Database Link	Entrez Gene 3791 Human UniProt ID P35968
Background	The vascular endothelial growth factor receptor-2 (VEGFR-2) plays an important role in the control of angiogenesis. This molecule is expressed on the surface of circulating endothelial stem cells as well as on adult vascular endothelial cells and other cell types (1). It is a 200-kDa type I transmembrane protein, consisting of a 19 amino acid leader peptide, a 744 amino acid extracellular domain made up of 7 immunoglobulin-like domains, a 24 amino acid transmembrane region and a 568 amino acid cytoplasmic domain. Interactions with VEGF ligands will stimulate angiogenesis, required in wound healing but also misused by tumours to promote their vascularization (2). The current monoclonal antibody was developed by genetic immunization against the complete extracellular domain of human VEGFR-2 and has been shown to be specific for the native target molecule and its counterpart in pigs using FACscan analyses on viable, transiently-transfected human BOSC23 cells (see Fig. 1). It binds to an epitope on the third extracellular immunoglobulin-like domain.
Synonyms	VEGFR2, FLK1, KDR, VEGF Receptor 2
Reference Data

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