MAPT / TAU pThr181 (177-187) Mouse Monoclonal Antibody [Clone ID: 2A4]

Specifications

Product Data
Clone Name	2A4
Applications	WB
Recommend Dilution	Indirect ELISA: The antibody reacts withphosphorylated peptide [Ala-Pro-Lys-Thr(p)-Pro-Pro-Ser-Ser-Gly-Glu-Cys] coated ELISA plate in indirect ELISA. The antibody cross- reacts with unphosphorylated peptide. Western blot: The antibody detected a 46kD band in mouse brain tissue lysate.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	KLH conjugated with a short peptide corresponding to the amino acid sequence 177-187 on the phosphorylated Tau protein
Specificity	This antibody recognizes the aa177-187 on phosphorylated Tau protein and its KLH conjugate, but not recognizes KLH alone. The antibody detected a 46kD band in mouse brain tissue lysate.
Formulation	0.01M PBS pH7.2 State: Aff - Purified State: Lyophilized Ig fraction
Reconstitution Method	Double distillated water is recommended to reconstitute the antibody.
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	microtubule associated protein tau
Database Link	Entrez Gene 4137 Human UniProt ID P10636
Background	Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.
Synonyms	MAPTL, MTBT1, Microtubule-associated protein tau, PHF-tau, Neurofibrillary tangle protein, Paired helical filament-tau
Reference Data

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