Complement C3 (C3) (C-term) Mouse Monoclonal Antibody [Clone ID: 755]

Specifications

Product Data
Clone Name	755
Applications	ELISA, WB
Recommend Dilution	Immunoassay. Western blot: A non-reduced or reduced sample treatment and SDS-PAGE was used. The band size (s) are ~190 and ~100 kDa under non-reducing and reducing conditions respectively. (Ref.1). The typical starting working dilution is 1:50. Not suitable for Functional assays. Positive control: Human serum. Negative control: C3 deficient serum.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Native C3
Specificity	The monoclonal antibody 755 recognizes an epitope located in the C-terminal 360 amino acids on the alpha chain of C3, thereby recognizing C3b and full C3. It does not cross react with Human C3a.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	complement component 3
Database Link	Entrez Gene 718 Human UniProt ID P01024
Background	The complement system is an important factor in innate immunity. The third complement component, C3, is central to the classical, alternative and lectin pathways of complement activation. The synthesis of C3 is tissue-specific and is modulated in response to a variety of stimulatory agents. C3 is the most abundant protein of the complement system with serum protein levels of about 1.3 mg/ml. An inherited deficiency of C3 predisposes a person to frequent bacterial infections. C3 fragments are deposited in tissues at sites of antibody-mediated immunopathology. In ulcerative colitis and idiopathic chronic inflammatory bowel disease, the deposition of C3 in the diseased mucosa has been reported. After activation of the complement system, certain enzymes become active, resulting in the cleavage of C3 into C3b and the anaphylatoxin C3a. C3b becomes attached to immune complexes and is further cleaved into iC3b, C3c, C3dg and C3f. Within the alternative pathway of complement, C3b plays a critical role in the amplification loop initiated by spontaneous hydrolysis of C3.
Synonyms	CPAMD1, Complement component 3
Reference Data

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