CD46 (SCR2 Dom.) Mouse Monoclonal Antibody [Clone ID: M177]

Specifications

Product Data
Clone Name	M177
Applications	ELISA, FN, IP, WB
Recommend Dilution	Flow Cytometry: Stains the extracellular domain of CD46 (Ref.1,2). The typical starting working dilution is 1/10. Functional Assays (Ref.1,3,5-7). Immunoassays (Ref.1). Immunoprecipitation (Ref.1,3,4): 107 cells were lysed and immunoprecipitated with 25 µg M177 antibody and 25µg protein G-sepharose. Western Blot: A non-reduced sample treatment and SDS-page was used. The band size is 45-70 kDa (Ref.1 and 2). The typical starting working dilution is 1/10. Positive Control: CD46 is expressed on every cell and tissue. Negative Control: Erythrocytes.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified MCP
Specificity	The monoclonal antibody M177 recognizes CD46, also designated membrane cofactor protein (MCP). The monoclonal antibody M177 reacts with the SCR2 domain.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD46 molecule
Database Link	Entrez Gene 4179 Human UniProt ID P15529
Background	CD46 is a 45-70 kDa protein with genetic and tissue-specific heterogeneity. It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to inhibit complement activation on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR).
Synonyms	TLX, MIC10
Reference Data

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