CHRM2 (168-192) Mouse Monoclonal Antibody [Clone ID: B8E5]

Specifications

Product Data
Clone Name	B8E5
Applications	ELISA, FN, IF, IHC, WB
Recommend Dilution	Flow Cytometry. Functional Assays (Agonist-like antibody). Immunoassay. Immunofluorescence. Western blot (Non-Reduced Conditions, specific bands of ~55, 63 and 65 kDa). The typical starting working dilution is 1/50.
Reactivity	Guinea Pig, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human second extracellular loop residues 168-192
Specificity	The monoclonal antibody B8E5 recognizes Human muscarinic acetylcholine receptor M2 (M2 receptor), a G protein-coupled cardiovascular receptor of ~55 kDa. Monoclonal antibody B8E5 inhibits the ß-adrenergic L-type Ca2+ currents through activation of the muscarinic acetylcholine receptor M2. It suggests that the antibody acts not via the classical pathway of decreasing cAMP, but rather by increasing cGMP. Monoclonal antibody B8E5 acts by functional dimerization of the receptor resulting in stabilization of the constitutive active receptor dimers and paradoxically induces a small decrease in carbachol affinity for the M2 receptor. It recognizes the pentapeptide VRTVE (aa 168-172) corresponding to the N-terminal part of the second extracellular loop of the Human M2 receptor.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% BSA
Concentration	lot specific
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C.
Gene Name	cholinergic receptor muscarinic 2
Database Link	Entrez Gene 1129 Human UniProt ID P08172
Background	This receptor is an integral membrane protein consisting of seven membrane spanning a-helices linked together by extra- and intracellular loops that form a pharmacophore pocket. Autoantibodies directed against cardiovascular G protein-coupled receptors functionally interfering with the target have been described in several cardiovascular diseases. The M2 receptor is the predominant sybtype of muscarinic receptors present in the heart of mammalian species. The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.
Synonyms	CHRM2, mAChR M2, mAChR-M2
Reference Data

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