MD2 (LY96) Mouse Monoclonal Antibody [Clone ID: 4H1]
CAT#: AM26186PU-N
MD2 (LY96) mouse monoclonal antibody, clone 4H1, Purified
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CNY 5,494.00
货期*
5周
规格
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Specifications
Product Data | |
Clone Name | 4H1 |
Applications | ELISA, FN, IF, WB |
Recommend Dilution | Functional assays: Inhibit LPS binding to MD-2. Immunoassay. Immunoflourescence. Western blot. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Baculovirally expressed His-tagged human MD-2 |
Specificity | This antibody reacts with soluble MD-2 (sMD-2) and the sMD-2/Toll-like receptor 4 (TLR4, CD284) complex. It reacts with both the monomeric and the polymeric form of sMD-2. |
Formulation | PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin |
Concentration | lot specific |
Purification | Protein G |
Conjugation | Unconjugated |
Storage Condition | Store at 2 - 8 °C. |
Gene Name | lymphocyte antigen 96 |
Database Link | |
Background | TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4. MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomer and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells. |
Synonyms | Lymphocyte antigen 96, ESOP-1, LY96, ESOP1, MD2 |
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