Complement factor B (CFB) Mouse Monoclonal Antibody [Clone ID: M13/12]

Specifications

Product Data
Clone Name	M13/12
Applications	ELISA, IP, WB
Recommend Dilution	Immunoassay. Western blot (typical starting working dilution is 1:50). Immunpoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This antibody detects Complement factor B/Bb.
Formulation	PBS State: Purified State: Liquid 0.2 µm filtered Ig fraction Stabilizer: 0.1% bovine serum albumin Preservative: 0.02% sodium azide
Concentration	lot specific
Purification	Protein G
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C.
Gene Name	complement factor B
Database Link	Entrez Gene 629 Human UniProt ID P00751
Background	Factor B is an acute-phase protein. Levels of factor B increase during inflammation. Complement factor B is a single- chain molecule of 764 amino acids (MW of 90 kD), including a leader peptide of 25 amino acids. Complement factor B provides the catalytic subunit of the C3/C5 convertases of the alternative complement pathway. Assembly of the C3 convertase (C3bBb) requires binding of complement factor B to C3b (C3.H2O) and factor D-mediated cleavage of bound complement factor B resulting in the release of Ba (MW 33 kD). The C3 convertase is stabilized by the binding of properdin. This provides a positive amplification loop for the classical and alternative complement pathways. Bb (MW 60 kD) is the serine protease element of this convertase. After cleavage of C3, the C5 convertase ((C3b)2Bb) is formed. The Bb fragment may be regarded as a better indicator of the alternative pathway of complement activation than Ba as impaired renal filtration does directly influence Ba levels. Whereas Bb is elevated 2.2-fold in chronic renal failure (CRF) patients, plasma concentrations of Ba are 8.4-fold higher in CRF and 16-fold higher in end-stage renal disease (ESRD) patients compared to normals.In addition to complement activation, factor B fragments participate in other immunological functions.
Synonyms	Properdin factor B, C3/C5 convertase, PBF2, CFB, BF, BFD
Reference Data

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