CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: c127]

Specifications

Product Data
Clone Name	c127
Applications	FC, IHC, IP
Recommend Dilution	Immunohistochemistry on Frozen Sections (Reacts mainly with granulocytes): 0.5 µg/ml (1/800). Has been described to work in FACS and Immunoprecipitation. Suggested Positive Control: Human placenta.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	The antibody recognizes CD16, the low affinity receptor for IgG (FcγRIII). It detects both isoforms. The antigen is strongly expressed on granulocytes in most tissues. Antigen Distribution: Isolated cells: On flow cytometry it stains approximately 2-15% of peripheral blood lymphocytes. Tissue Sections: The antigen is strongly expressed on granulocytes in most tissues.
Formulation	Stock solution contains 0.4 mg/ml IgG, Phosphate Buffered Saline pH 7.2 (PBS) State: Aff - Purified State: Lyophilized purified Ig fraction Stabilizer: 5 mg/ml BSA Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore by adding 0.5 ml distilled water.
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Fc fragment of IgG receptor IIIa
Database Link	Entrez Gene 2214 Human UniProt ID P08637
Background	CD16 is expressed on the majority of NK cells, on granulocytes, monocytes and macrophages, and on a subset of T cells. CD16 is involved in antibody-dependent cellular cytotoxicity (ADCC). This molecule is encoded by FcγRIII-A or FcγRIII-B genes. The genetic heterogeneity of FcγRIII generates alternative membrane-anchored proteins with distinct signalling capacities when cross-linked by immune complexes. CD16 exists in two distinct forms, as a single-pass type I integral membrane protein (CD16a), and as a glycosyl phosphatidyl inositol (GPI) form (CD16b). In humans, the transmembrane form is expressed on NK cells and macrophages while the GPI linked form is expressed on granulocytes.
Synonyms	FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
Reference Data
Protein Families	ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways	Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus

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