CD19 Mouse Monoclonal Antibody [Clone ID: AE1]

Specifications

Product Data
Clone Name	AE1
Applications	FC, IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1-3 μg/ml (1/50-1/200). Does not react on routinely processed paraffin sections. Has been described to work in FACS. Suggested Positive Control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	This Monoclonal antibody AE1 recognizes the CD19 antigen also known as Leu-12.  Antigen Distribution:   Isolated cells: 5-50% of human peripheral blood mononuclear cells. Tissue sections: The antigen is expressed on B-cells and follicular dendritic cells.
Formulation	Stock solution contains Phosphate buffered saline pH 7.2 (PBS), 5 mg/ml BSA as a stabilizer and 0.09% Sodium Azide as a preservative State: Aff - Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore by adding 0.5 ml distilled water.
Concentration	0.2 mg/ml IgG (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	CD19 molecule
Database Link	Entrez Gene 930 Human UniProt ID P15391
Background	CD19, also known as Leu-12, is expressed on B cells and follicular dendritic cells. CD19 is a signal transduction molecule which regulates B-cell development, activation, and differentiation. The CD19 antigen appears early in B lymphocyte differentiation at approximately the same stage as the CD22 antigen. CD19 is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression.
Synonyms	Leu-12, B-cell marker
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	B cell receptor signaling pathway, Hematopoietic cell lineage, Primary immunodeficiency

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