FLT3 Mouse Monoclonal Antibody [Clone ID: BV10A4]

Specifications

Product Data
Clone Name	BV10A4
Applications	FC, IP
Recommend Dilution	Flow cytometry: Tested on cell lines K562 and REH. In this case the recommended concentration is 10 μg/ml per 1 million cells/ml. Immunoprecipitation.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	BV-173 leukemic cell line
Specificity	This antibody reacts with CD135 (FLT3, FLK2, STK-1), a 130-160 kDa type III receptor tyrosine kinase that is involved in early steps of hematopoiesis.
Formulation	PBS, pH 7.4 containing 0,05% sodium azide as stabilizer State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	fms related tyrosine kinase 3
Database Link	Entrez Gene 2322 Human UniProt ID P36888
Background	CD135 / FLT3, also known as FLK2 or STK-1 is a receptor tyrosine kinase that plays important roles in hematopoiesis. After binding of Flt3 ligand (FL), CD135 homodimerizes and stimulates proliferation, differentiation and protects the cell from apoptosis. The loss of CD90 and gain of CD135 expression marks the loss of self-renewal in hematopoietic stem cell population. Detectable CD135 expression appears first at low levels on the surface of primitive multilineage progenitor cells and disappears during defined stages of B-cell development, but is upregulated and maintained during maturation of monocytes. CD135 is also expressed on thymocytes, dendritic cell progenitors and on mature dendritic cells, as well as on various malignant hematopoietic cells.
Synonyms	FL cytokine receptor, STK1
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
Protein Pathways	Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

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