E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: 67A4]

Specifications

Product Data
Clone Name	67A4
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Flow cytometry: Tested on cell lines CACO-2 and HT-29. In this case the recommended concentration is 5-10 μg/ml per 1 million cells/ml. Immunoprecipitation. Western blot. Immunohistochemistry (frozen sections): 4-8 μg/ml; positive tissue: tonsil. Immunocytochemistry.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	T-47D cells
Specificity	This antibody recognizes CD324 / E-cadherin, an approximately 100 kDa epithelial cell adhesion molecule, whose detection is important for determination of invasive potential of epithelial neoplasms.
Formulation	Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Gene Name	cadherin 1
Database Link	Entrez Gene 999 Human UniProt ID P12830
Background	CD324 / E-cadherin is an epithelial cell surface molecule, which provides calcium-dependent homophilic interactions with E-cadherin of another cell. These intaractions take part in morphogenetic programs controlling the maintenance of the structural and functional integrity of epithelia and affect invasive potential of epithelial neoplasms. CD324 / E-cadherin is implicated in cell growth and differentiation, cell recognition, and sorting during developmental morphogenesis, as well as in aggregation-dependent cell survival. CD324 / E-cadherin-mediated cell adhesion system is highly regulated from inside the cell by a number of intracellular signaling pathways.
Synonyms	Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
Reference Data

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