GST-Tag Mouse Monoclonal Antibody [Clone ID: 12G8]

Specifications

Product Data
Clone Name	12G8
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1/5000. Incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1 TBS, 0.05% Tween-20 at 4°C with gentle shaking, overnight. ELISA: 1/2000.
Reactivity	All Species, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	This monoclonal antibody is produced by immunizing animals with full-length GST.
Specificity	This GST-Tag Antibody (12G8) Mouse mAb detects GST and GST fusion proteins.
Formulation	State: Aff - Purified State: Liquid purified Ig fraction
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08515
Background	Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.
Synonyms	Glutathione S-transferase Tag, GST26-Tag
Reference Data

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