Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: CK-7]

Specifications

Product Data
Clone Name	CK-7
Applications	IHC, WB
Recommend Dilution	Western Blot: 1 - 2 μg/ml. Immunohistochemistry on frozen and paraffin sections: 2 - 4 μg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal preparation of the RT4human bladder carcinoma cell line.
Specificity	This antibody reacts to Cytokeratin 7.
Formulation	1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative. State: Purified State: Lyphilized purified Ig fraction
Reconstitution Method	Restore with 1.2% sodium acetate or neutral PBS
Concentration	0,1 mg/ml (after reconstitution with PBS)
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at -20°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	keratin 7
Database Link	Entrez Gene 3855 Human UniProt ID P08729
Background	KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73% homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans.
Synonyms	KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin
Reference Data
Protein Families	ES Cell Differentiation/IPS

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