pan Actin Mouse Monoclonal Antibody [Clone ID: AC-40]

Specifications

Product Data
Clone Name	AC-40
Applications	IF, IHC, WB
Recommend Dilution	Western blot: Use at 0.1-0.5 μg/ml with the appropriate system to detect Actin in cells and tissues. Immunohistochemistry on Frozen Sections: Use at 0.5-1.0 μg/ml to detect Actin in Formalin/Acetone fixed tissues. Immunocytochemistry. 1.0 μg/ml
Reactivity	Chicken, Human, Mouse, Rat, Snake
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic Actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.
Specificity	Recognizes Actin. No cross reactivity with other proteins.
Formulation	1.2% Sodium Acetate containing 2 mg BSA and 0.01 mg Sodium Azide as preservative. State: Aff - Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1.2% Sodium Acetate or neutral PBS.
Concentration	0.1 mg/ml (after reconstitution with 1 ml PBS).
Purification	Goat anti-Mouse IgG Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. After reconstituion store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.
Reference Data

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