MET (Cytopl. Dom.) Mouse Monoclonal Antibody [Clone ID: IST-1]

Specifications

Product Data
Clone Name	IST-1
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 1/50. Immunohistochemistry on Paraffin Sections: 10 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide derived from cytoplasmic domain of Human c-Met protein
Specificity	Recognizes c-Met (Cytoplasmic Domain).
Formulation	PBS, 7.4 containing 1% BSA and 0.09% Sodium Azide State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	MET proto-oncogene, receptor tyrosine kinase
Database Link	Entrez Gene 4233 Human UniProt ID P08581
Background	The c-Met protein is a receptor tyrosine kinase encoded by the c-Met proto-oncogene. c-Met, also known as HGF receptor or SF receptor, is activated by hepatocyte growth factor (HGF) or scatter factor (SF), and is composed of alpha and beta subunits linked by disulfide bonds. Dysregulated c-Met expression has been detected at both protein and mRNA levels in a variety of human carcinomas and sarcomas. Up to a 2.5-fold increase in mRNA expression has been reported in colorectal tumor tissues, and upregulation of the c-Met protein in association with increasingly malignant behavior has been described in prostate cancer, cutaneous malignant melanoma, and breast cancer. Recent studies using monoclonal Ms anti-c-Met (clone: 3D4) for immunohistochemistry in lymph node-negative breast carcinoma tissues demonstrated that high levels of c-Met expression are associated with poor patient clinical outcome, as assessed over 20-40 years of follow-up. Further, antibodies against the intracellular but not the extracellular domain of c-Met were prognostic, suggesting that overexpression of the cytoplasmic tail of c-Met may play an important role in breast cancer progression.
Synonyms	Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma

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