TRITC Mouse Monoclonal Antibody [Clone ID: NI 308]

Specifications

Product Data
Clone Name	NI 308
Applications	ELISA, IF, WB
Recommend Dilution	To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. General Recommended Dilutions: Histochemical Use: 1/10-1/40. ELISA: from 1/100 upwards (Incubation times for the antibody are approx. 1 hour). Western blot: from 1/200 upwards.
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified Tetramethylrhodamine Isothiocyanate isomer R.
Specificity	The reactivity of the antiserum is directed to TRITC as tested in direct binding enzyme immunoassay, ELISA and Immunoblotting. This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
Formulation	PBS, pH 7.2 without foreign proteins. State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.
Synonyms	Tetramethylrhodamine Isothiocyanate Isomer 1
Reference Data

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