Human Lambda Light Chain (free and bound) Mouse Monoclonal Antibody [Clone ID: NI 412, NI 268]

Specifications

Product Data
Clone Name	NI 412, NI 268
Applications	ELISA, IF, IP, WB
Recommend Dilution	To identify the light chain type of immunoglobulins or free light chains in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, Indirect Immunoperoxidase staining and immunoblotting. General Recommended Dilutions: Histochemical Use: 1/10-1/100 ELISA: from 1/50 upwards Western blot: from 1/100 upwards.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly purified Bence Jones lambda proteins isolated from pooled Human urine.
Specificity	The antibody does not react with any other component of the human immunoglobulin system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species. The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the lambda type, as well as free lambda light chains as tested in direct binding enzyme immunoassay, Immunoblotting, Immunoprecipitation and direct immunoperoxidase staining.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: HRP State: Lyophilized purified IgG fraction Label: Horseradish Peroxidase Enzyme marker: Horseradish Peroxidase enriched for isoenzyme C (RZ=3.2) Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life Molar radio: Peroxidase/IgG: ~1.7
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water.
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	HRP
Storage Condition	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.
Reference Data

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