Dityrosine / DT Mouse Monoclonal Antibody [Clone ID: 1C3]

Specifications

Product Data
Clone Name	1C3
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA (Ref.1,2) Western blot: 4 µg/ml (Ref.3,5) Immunohistochemistry on Paraffin Sections (Ref.4,6).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	3-(p-hydroxyphenyl) Propionic Acid Dimer, KLH-conjugated
Specificity	Specific for free Dityrosine, 3-(p-hydroxyphenyl) Propionic Acid dimer, Dityrosine-BSA conjugate, and Dityrosine in proteins or peptides. There was no cross reactivity detected for following compounds: Tyrosine, Nitrotyrosine, Chlorotyrosine, Aminotyrosine, Phosphotyrosine, 3-(phydroxyphenyl) Propionic Acid, Phenylalanine, 3,4-Dihydroxyphenylacetic Acid (DOPA).
Formulation	10mM PBS, pH 7.4 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 0.2 ml aqua bidest to 0.1 mg/ml of IgG
Concentration	0.1 mg/ml (after reconstitution)
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Background	Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.
Reference Data

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