Raptor (RPTOR) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10E10]

Specifications

Product Data
Clone Name	10E10
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for more details).
Reactivity	Canine, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Peptide conjugated to hemocyanin derived from Raptor Sequence.
Specificity	Recognizes Raptor.
Formulation	1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name	regulatory associated protein of MTOR complex 1
Database Link	Entrez Gene 74370 MouseEntrez Gene 287871 RatEntrez Gene 57521 Human UniProt ID Q8N122
Background	The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473
Synonyms	RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycin TOR-scaffold protein
Note	Mol. weight: 145 kDa Protocol: Positive Control Provided: Cell lysate from untreated Hela cells Description: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human) Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Pathways	Insulin signaling pathway, mTOR signaling pathway

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