MET (Activation Loop) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 14G9]

Specifications

Product Data
Clone Name	14G9
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for more details).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	peptide conjugated to hemocyanin Epitope: kinase activation loop.
Specificity	This monoclonal MET antibody (14G9) recognizes Met activation loop.
Formulation	1ml PBS, containing 0.09% Sodium Azide/PEG and Sucrose. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	For long-term storage, freeze lyophilizate upon arrival (-20°C). Upon reconstitution, aliquote and freeze in liquid nitrogen; reconstituted antibody can be stored frozen at -80°C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name	MET proto-oncogene, receptor tyrosine kinase
Database Link	Entrez Gene 4233 Human UniProt ID P08581
Background	MET is a high affinity receptor for Hepatocyte Growth Factor/Scatter Factor HGF/SF. The (HGF/SF)-(HGFR/MET) ligand-receptor system appears to have an important role in controlling interaction between mesenchymal cells and other cell types and has been implicated in the cell mitogenic response, cell motility and development. The interaction with HGF leads to autophosphorylation at multiple tyrosine residues and recruiting of several downstream signaling components (Gab1, c-cbl, PI3-kinase). The phosphorylation of Tyr 1234/1235 leads to kinase activation, and phospho-Tyr 1349 is the Gab1 binding site. Altered MET levels and/or tyrosine kinase activities have been found in different tumors, such as colon, renal, and breast cancer.
Synonyms	Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met
Note	Mol. weight: 145 kDa Protocol: Positive Control Provided. Cell lysate from untreated A431 cells Description: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human) Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution:Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
Protein Pathways	Adherens junction, Axon guidance, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Epithelial cell signaling in Helicobacter pylori infection, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.