GSK3 alpha (GSK3A) pTyr278/215 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6D3]

Specifications

Product Data
Clone Name	6D3
Applications	WB
Recommend Dilution	Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from pervanadate treated HepG2 cells (See Protocols for more details).
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Phosphopeptide conjugated to hemocyanin Epitope: Phosphotyrosine 215, Phosphotyrosine 278
Specificity	This antibody specifically recognizes GSK3beta phosphorylated at Tyr215 at 46 kDa and GSK3alpha phosphorylated at Tyr278 at 54 kDa in Western blot applications.
Formulation	1 ml PBS with 0.09% Sodium Azide/PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with 1 ml H2O (15 min, RT).
Purification	Subsequent Ultrafiltration and Size Exclusion Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
Gene Name	glycogen synthase kinase 3 alpha
Database Link	Entrez Gene 606496 MouseEntrez Gene 2931 Human UniProt ID P49840
Background	Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.
Synonyms	Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A
Note	Mol. weight: 46 kDa Protocol: Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cells Description: Cell lysate from pervanadate-treated HepG2 cells, hepatocellular carcinoma (Human) Format: Lyophilized cell lysate from from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Chemokine signaling pathway

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