ATG5 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11C3]

CAT#: AM20206PU-N

ATG5 (incl. pos. control) mouse monoclonal antibody, clone 11C3, Purified



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CNY 5,973.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 11C3
Applications WB
Recommend Dilution Immunobotting (Western Blot): 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blot incubation buffer.
Included Postitive Control: Cell lysate from untreated SH-SY5Y cells (See Protocols for more details).
Reactivity Canine, Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Immunogen Recombinant ATG5
Specificity Recognizes the ATG5-ATG12 protein complex at 55 kDa in immunoblot application.
Formulation PBS
State: Purified
State: Lyophilized purified IgG fraction
Stabilizer: PEG and Sucrose
Preservative: 0.09% Sodium Azide
Reconstitution Method Restore with 1ml H2O (15 min, RT).
Purification Purified from Serum-Free Cell Culture Supernatant by Subsequent Ultrafiltration and Size Exclusion Chromatography
Conjugation Unconjugated
Storage Condition Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C.
Avoid repeated freezing and thawing.
Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
Gene Name autophagy related 5
Background Autophagy as a response to cellular stress and starvation is an alternative process of proteasomal degradation for some long-lived proteins and organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). The gene product of autophagy-related gene 5 (ATG5) is required for autophagosome-formation. ATG5 also enhances the susceptibility towards apoptotic stimuli. Like Bcl-2, ATG5 exhibits a dual function by modulating both autophagy and apoptosis.
Synonyms ASP, APG5-like, Autophagy protein 5
Note Molecular Weight: 24, 33, 55 kDa

Protocol: Positive Control: Cell lysate from untreated SH-SY5Y cells.
Formulation: Lyophilized cell lysate from Serum starved SH-SY5Y cells.
Stability:
Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
Application:
The Positive Control lysate is recommended for Immunoblot applications. 20µl of Positive Control correspond to ca. 20.000 cells.
Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
Storage:
Aliquote and store frozen.
Avoid repeated freeze/thaw cycles.
Shelf life: one year from despatch.
The Lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as Immunoprecipitation.
Reference Data
Protein Families Druggable Genome
Protein Pathways Regulation of autophagy, RIG-I-like receptor signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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