Cd3e Hamster Monoclonal Antibody [Clone ID: 145-2C11]

Specifications

Product Data
Clone Name	145-2C11
Applications	FC, FN, IF, IHC, IP
Recommend Dilution	Flow Cytometry: 1-2 μg / ml (million cells). Immunoprecipitation: 1-2 μg / 100-500 μg protein in 1 ml cell lysate. Immunohistochemistry (frozen sections). Immunocytochemistry. Functional Application: Induction of T cell activation, proliferation or apoptosis (depending on conditions); in vivo T cell depletion.
Reactivity	Mouse
Host	Hamster
Clonality	Monoclonal
Immunogen	Mouse BM10-37 cytotoxic T lymphocytes
Specificity	This antibody reacts with mouse CD3 (epsilon subunit). It is commonly used as a phenotypic marker for mouse T cells.
Formulation	Phosphate buffered saline (PBS), approx. pH 7.4 State: Low Endotoxin State: Liquid Ig fraction
Concentration	lot specific
Purification	Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	CD3 antigen, epsilon polypeptide
Database Link	Entrez Gene 12501 Mouse UniProt ID P22646
Background	CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Synonyms	T3/Leu-4
Reference Data

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