PAX8 Rabbit Monoclonal Antibody [Clone ID: ZR-1]

Specifications

Product Data
Clone Name	ZR-1
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/100 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval (EDTA, pH 9.0) is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Ovarian serous carcinoma.
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to the C-terminus of Human PAX8 protein.
Specificity	Recognizes Pax8. Cellular Localization: Nuclear.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant Stabilizer: 0.2% BSA Preservative: 15mM Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	paired box 8
Database Link	Entrez Gene 4747 HumanEntrez Gene 7849 Human UniProt ID Q06710
Background	PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. Studies have also found PAX-8 experession in renal tubules as well as renal carcinoma, nephroblastoma and seminoma. Over 98% of clear cell RCCs, 90% of papillary RCCs, and 95% of oncocytomas were positive for PAX-8, frequencies which are similar or better than for PAX-2. Normal lung and lung carcinomas do not express PAX-8. Similarly, the absence of expression of PAX-8 in breast and other non-GYN carcinomas other than those primary to the thyroid indicates that PAX-8 is an important new marker of ovarian cancer and a useful marker for the differential diagnoses in lung and neck tumors, or tumors at distant sites where primary lung carcinoma or thyroid carcinoma are possibilities. PAX-8, combined with organ system-specific markers such as uroplakin, mammaglobin, and TTF-1 can be a very useful panel to determine the primary site of invasive micropapillary carcinomas of ovary from bladder, lung, and breast.
Synonyms	Pax-8
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways	Amyotrophic lateral sclerosis (ALS)

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