MLH1 Mouse Monoclonal Antibody [Clone ID: G168-728]

Specifications

Product Data
Clone Name	G168-728
Applications	IHC
Recommend Dilution	Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Colon carcinoma.
Reactivity	Hamster, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant full length MLH protein.
Specificity	The G168-15 antibody recognizes the Human and Mouse MLH1 (80-85kDa). Cellular Localization: Nuclear.
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	mutL homolog 1
Database Link	Entrez Gene 17342 MouseEntrez Gene 25094 RatEntrez Gene 4292 Human UniProt ID P40692
Background	The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.
Synonyms	DNA mismatch repair protein Mlh1, COCA2
Reference Data
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Melanogenesis, Melanoma, Pathways in cancer

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