pan Cytokeratin Mouse Monoclonal Antibody [Clone ID: cocktail]

Specifications

Product Data
Clone Name	cocktail
Applications	FC, IF
Recommend Dilution	Immunofluorescence: 10-20 µg/ml (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4°C. Flow Cytometry: 0.2-1.0 µg/0.1 ml (1/200-1/1,000) (Not tested in our lab).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human epidermal keratin.
Specificity	This antibody recognizes cytokeratins CK1 (67), CK2 (65.5), CK3 (64), CK4 (59) CK5 (58), CK6 (56), CK8 (52.5), CK10 (56.5), CK13 (54) CK14 (50), CK15 (50), CK16 (48), CK18 (45), CK 19 (40). Cellular Localization: Cytoplasmic.
Formulation	PBS, pH 7.4 containing 1% BSA as stabilizer and 0.05% Sodium Azide as preservative. Label: FITC State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Background	Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1(67), CK2(65.5), CK3(64), CK4(59), CK5(58), CK6(56), CK7(54), CK8(52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16 (48), CK17 (46), CK18 (45), CK19 (48) and CK20 (46).
Synonyms	pan Keratin, Cytokeratin pan-reactive
Reference Data

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