RAF1 (N-term) Mouse Monoclonal Antibody [Clone ID: 540]

Specifications

Product Data
Clone Name	540
Applications	ELISA, WB
Recommend Dilution	ELISA. Western Blot: The antibody, when used at 1 μg/ml, will allow visualization of 0.2 μg/lane of recombinant Human Raf-1 (75 kDa).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Raf-1 (mw:75 kDa)
Specificity	This antibody is specifically reactive to Raf-1 N-terminal (1-240 a.a.)
Formulation	0.01M PBS, pH 7.2 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with double distillated water to adjust the final concentration to 1,0 mg/mL
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Prior to and following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	Raf-1 proto-oncogene, serine/threonine kinase
Database Link	Entrez Gene 5894 Human UniProt ID P04049
Background	The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
Synonyms	C-RAF, Raf-1, cRaf
Reference Data
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway

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