PSMA (FOLH1) Mouse Monoclonal Antibody [Clone ID: 4H11]

Specifications

Product Data
Clone Name	4H11
Applications	ELISA, IHC
Recommend Dilution	ELISA. Immunohistochemistry on Paraffin Sections: PSMA were detected in Paraffin-Embedded Human prostatic tissue and Prostatic Carcinoma tissue with Monoclonal PSMA antibody (Cat#AM09256PU-N, Clone: 4H11).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Crude membrane protein extraction from PSMA expression LNCap cells, and a PSMA derived peptide (ESKVDPSKA) coupled with KLH.
Specificity	This antibody is reactive to PSMA expression LNCap cells and the PSMA peptide in EIA. In IHC staining experiment, this antibody reacts to human prostatic carcinoma tissue, but not to normal prostatic tissue. Little or no cross-reactivity to normal prostatic tissue.
Formulation	0.01M PBS, pH 7.0 without preservatives. State: Aff - Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with Double distillated water to adjust the final concentration to 1.0 mg/ml.
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	folate hydrolase (prostate-specific membrane antigen) 1
Database Link	Entrez Gene 2346 Human UniProt ID Q04609
Background	Prostate Specific Membrane Antigen (PMSA, FOLH1) is a type II transmembrane glycoprotein belonging to the M28 peptidase family. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. A mutation in the intestinal form may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. The form expressed in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. The prostate form is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.
Synonyms	Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen, FOLH, NAALAD1, PSM, GCP2, NAALAdase
Reference Data
Protein Families	Druggable Genome, Protease, Transmembrane

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.