Interferon gamma (IFNG) Mouse Monoclonal Antibody [Clone ID: 315]

Specifications

Product Data
Clone Name	315
Applications	ELISA
Recommend Dilution	ELISA: This IFN gamma antibody can be used as a Capture Antibody in Sandwich ELISA applications for Human IFN-gamma Detection in combination with a monoclonal Tracer/Detection antibody (HRP-conjugated, Cat#AM09176HR-N). Suggested Capture Coating Dose: 0.3 µg/well. Substrate: TMB. If the above suggested conditions are followed approximately 13.1 pg/mL of IFN-gamma in serum/plasma or 7.5 pg/ml of IFN-gamma in cell medium/urine can be detected with an assay range of 0-1600 pg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant Human Interferon gamma (IFN gamma).
Specificity	This antibody reacts with natural and recombinant Human IFN-gamma.
Formulation	0.01M PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified IgG fraction.
Reconstitution Method	Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/ml
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody at -20°C. Avoid repeated freezing and thawing.
Gene Name	interferon, gamma
Database Link	Entrez Gene 3458 Human UniProt ID P01579
Background	Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms	IFN-gamma, gamma IFN
Reference Data
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Allograft rejection, Cytokine-cytokine receptor interaction, Graft-versus-host disease, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, Proteasome, Regulation of autophagy, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Type I diabetes mellitus

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