CD45 (PTPRC) Mouse Monoclonal Antibody [Clone ID: BRA-55]

Specifications

Product Data
Clone Name	BRA-55
Applications	IF, IHC, WB
Recommend Dilution	Immunocytochemistry. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (Pretreatment not required). Recommended Dilutions: 1/10-1/20 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10x higher, but the optimal dilution should be tested by serial dilution). Dilution Buffer: PBS or TRIS.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated from REH6 cell line
Specificity	The antibody reacts with 170 - 220 kD cell surface glycoproteins (CD45), the Leukocyte Common Antigen (LCA), expressed selectively on hematopoietic cells. The monoclonal antibody LCA (CD45) is a useful marker to differentiate between lymphomas and carcinomas. Since this monoclonal gives a pronounced staining on formalin fixed paraffin embedded cells it is very useful in routine pathology.
Formulation	State: Ascites State: Liquid Ascites Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	protein tyrosine phosphatase, receptor type C
Database Link	Entrez Gene 5788 Human UniProt ID P08575
Background	CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.
Synonyms	PTPRC, Leukocyte common antigen, L-CA, T200
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway

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