ERK1 / ERK2 Mouse Monoclonal Antibody [Clone ID: SB46b]
CAT#: AM08205BT-N
ERK1 / ERK2 mouse monoclonal antibody, clone SB46b, Biotin
Conjugation: Unconjugated HRP
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CNY 4,312.00
Cited in 5 publications. |
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Specifications
Product Data | |
Clone Name | SB46b |
Applications | IP, WB |
Recommend Dilution | Western Blot. Predicted Mol. Weight: 42 and 44 kDa. Immunoprecipitation. |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Immunogen | Human recombinant Erk1 and Erk2. |
Specificity | This antibody precipitates ~42 kDa and ~44 kDa bands, corresponding to Erk1 and Erk2, respectively. |
Formulation | PBS containing 0.09% Sodium Azide as preservative. Label: Biotin State: Liquid purified Ig fraction. |
Concentration | lot specific |
Conjugation | Biotin |
Storage Condition | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Background | Erk1 and Erk2 are closely related mitogen activated protein (MAP) kinases which are activated by many growth factors, mitogens and differentiation-promoting agents via a protein kinase cascade. Also known as extracellular signal-regulated kinase 1 and 2, p44/p42 MAP kinases, microtubule-associated protein-2 kinases, myelin basic protein (MBP) kinases or EGF receptor T669 (Ert) kinases. (Ref.1-4) Erk1 and Erk2 are ubiquitous and abundant, although their relative abundance in specific tissues may vary. The two kinases are nearly 85% identical and have higher identity in the core regions involved in substrate binding. (Ref.4) Erk1 and Erk2 are activated approximately 1000-fold by phosphorylation within a Thr-Glu-Tyr motif in the activation loop on both threonine and tyrosine residues by Mek1 and Mek2. (Ref.4,5) Both sites must be phosphorylated for maximum activity. (Ref.1-4) These kinases in turn phosphorylate a variety of different substrates. Erk1 and Erk2 are found in the cytoplasm and are translocated to the nucleus upon activation. Erk1 and Erk2 target membrane proteins, cytosolic proteins, such as downstream kinases, and cytoskeletal proteins and nuclear proteins, such as transcription factors. Many of these substrates are important regulatory proteins. Erk1 and Erk2 represent proximal kinases in the classical kinase pathway which links growth and differentiation signals at the cell surface (through tyrosine kinases) with transcription in the nucleus. (Ref.1-3) |
Synonyms | ERK-1/ERK-2, MAPK1/MAPK2, P42/P44-MAPK |
Reference Data |
Citations (5)
The use of this Antibodies has been cited in the following citations: |
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Pharmaceutical inhibition of AXL suppresses tumor growth and invasion of esophageal squamous cell carcinoma
,null,
Experimental and Therapeutic Medicine
,PubMed ID 32952632
|
Minimal contribution of ERK1/2-MAPK signalling towards the maintenance of oncogenic GNAQQ209P-driven uveal melanomas in zebrafish
,null,
Oncotarget
,PubMed ID 27166257
|
The Kinase Activity-deficient Isoform of the Protein Araf Antagonizes Ras/Mitogen-activated Protein Kinase (Ras/MAPK) Signaling in the Zebrafish Embryo *
,null,
The Journal of Biological Chemistry
,PubMed ID 26306042
|
The Genomic Landscape of Response to EGFR Blockade in Colorectal Cancer
,null,
Nature
,PubMed ID 26416732
|
Novel cross-talk between IGF-IR and DDR1 regulates IGF-IR trafficking, signaling and biological responses
,null,
Oncotarget
,PubMed ID 25840417
|
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