GFAP Mouse Monoclonal Antibody [Clone ID: SB61b]

Specifications

Product Data
Clone Name	SB61b
Applications	IHC, WB
Recommend Dilution	Western Blot: 2 µg/ml Predicted Mol. Weight: 50 kDa. Immunohistochemistry (FS or Methanol fixed cells): 5 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human Glial Fibrillary Acid Protein (GFAP).
Specificity	This antibody reacts with human GFAP (~ 50 kDa).
Formulation	100 mM Borate buffered saline, pH 8.2 without preservatives. State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	glial fibrillary acidic protein
Database Link	Entrez Gene 2670 Human UniProt ID P14136
Background	Glial Fibrillary Acid Protein (GFAP) was discovered by Bignami et al. (Ref.1) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. (Ref.2) Many types of brain tumors, presumably of astrocytic origin, heavily express GFAP. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells of salivary tumors, and has been reported in erythrocytes. Therefore, antibodies to GFAP are very useful as markers of astrocytic cells and neural stem cells, and for distinguishing neoplasms of astrocytic origin from other neoplasms in the central nervous system. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein may also play a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the myelin layer that covers nerve cells. Additionally, GFAP may assist in maintaining the protective blood-brain barrier. In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in response to a variety of physical, chemical and etiological insults, including Alzheimer's disease, epilepsy and multiple sclerosis. Alexander's disease was recently shown to be caused by point mutations in the protein-coding region of the GFAP gene. (Ref.3) All forms of Alexander's disease are characterized by the presence of Rosenthal fibers, which are GFAP-containing cytoplasmic inclusions found in astrocytes.
Synonyms	Glial Fibrillary Acidic Protein
Reference Data

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