Bu-1b Mouse Monoclonal Antibody [Clone ID: 5-11G2]

Specifications

Product Data
Clone Name	5-11G2
Applications	FC
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivity	Chicken
Host	Mouse
Clonality	Monoclonal
Immunogen	Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.
Specificity	This antibody recognizes Chicken Bu-1b alloantigen (Mr. 70 kDa). It reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified Ig fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Database Link	UniProt ID Q90747
Background	Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
Synonyms	B6.2, chicken B-cell marker
Reference Data

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