Cd80 Rat Monoclonal Antibody [Clone ID: 1G10]

Specifications

Product Data
Clone Name	1G10
Applications	FC
Recommend Dilution	Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.2,7)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises CD80/B7-1 (Mr. 45-47 kDa). This Monoclonal CD80 antibody 1G10 blocks binding of CTLA-4 to CD80, but it does not block stimulation of T cells by natural antigenpresenting cells. (Ref.2,7).
Formulation	PBS containing 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified Ig fraction. Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD80 antigen
Database Link	Entrez Gene 12519 Mouse UniProt ID Q00609
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Synonyms	CD28LG, CD28LG1, LAB7, BB1, B7.1, B7-1
Reference Data

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