Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

Specifications

Product Data
Clone Name	53-6.7
Applications	FN
Recommend Dilution	Functional Assay: In vivo and in vitro depletion of CD8a+ cells. (Ref. 1,4,5) Fractionation of CD8a+ T cells. (Ref.10)
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Specificity	This antibody recognises the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Formulation	PBS without preservatives. State: Low Endotoxin State: Liquid purified Ig fraction.
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	CD8 antigen, alpha chain
Database Link	Entrez Gene 12525 Mouse UniProt ID P01731
Background	In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an alpha chain (CD8a/Lyt-2) and a beta chain (CD8b/Lyt-3); and (ii) a homodimer of two alpha chains. The heterodimer is found on the surface of essentially all thymocytes and the ''suppressor/cytotoxic'' subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b- T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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