E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: 7H12]

Specifications

Product Data
Clone Name	7H12
Applications	ELISA, FC, IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Flow Cytometry: 1/200 - 1/400. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human, Monkey, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of Human CDH1 expressed in E. Coli.
Specificity	Recognizes CDH1 (CD324 / Cadherin-1).
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	135 kDa
Gene Name	cadherin 1
Database Link	Entrez Gene 999 Human UniProt ID P12830
Background	E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.Tissue specificity: Non-neural epithelial tissues.
Synonyms	Epithelial cadherin, E-cadherin, Uvomorulin, CAM 120/80, CDH1, CDHE, UVO
Reference Data

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