NF-kB p65 (RELA) Mouse Monoclonal Antibody [Clone ID: 6H7]

Specifications

Product Data
Clone Name	6H7
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1/500 - 1/2000. ELISA: 1/10000.
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of human NF-κB p65 expressed in E. Coli.
Specificity	This antibody reacts to NF-kappaB p65.
Formulation	State: Ascites State: Ascitic fluid containing 0.03% sodium azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	65 kDa
Gene Name	RELA proto-oncogene, NF-kB subunit
Database Link	Entrez Gene 5970 Human UniProt ID Q04206
Background	Transcription factors of the nuclear factor κ B (NF-κB)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals: RelA (p65), c-Rel, RelB, NF-κB1 (p105/p50) and NF-κB2 (p100/p52). The most studied NF-κB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for theinteraction of NF-κB with its inhibitor, IκB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IκB. This complex prevents nuclear translocation and activity of NF-κB. In response to stimuli such as cytokines, LPS, and viral infections, IκB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IκB/NF-κB complex, allowing the free heterodimeric NF-κB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the κB site within promoters and enhancers and functions as a transcriptional activator.
Synonyms	NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3
Reference Data

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