PRAK (MAPKAPK5) Mouse Monoclonal Antibody [Clone ID: 7H10B4]

Specifications

Product Data
Clone Name	7H10B4
Applications	IHC, WB
Recommend Dilution	ELISA: 1/10000. Western Bloting: 1/500 - 1/2000. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of PRAK expressed in E. Coli.
Specificity	Recognizes PRAK (p38-regulated /activated kinase).
Formulation	State: Ascites State: Ascitic fluid containing 0.03% Sodium Azide.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase-activated protein kinase 5
Database Link	Entrez Gene 8550 Human UniProt ID Q8IW41
Background	PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
Synonyms	MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK
Reference Data

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