SIGLEC1 Mouse Monoclonal Antibody [Clone ID: 3B1/11]

Specifications

Product Data
Clone Name	3B1/11
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to labell 1x105 cells in 100 µl. Immunohistochemistry on Frozen Sections. Positive Control: Porcine spleen. Western Blot: Detects a band of approximately 190kD in alveolar macrophage extracts under non-reducing conditions.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine alveolar macrophages.
Specificity	This antibody is specific for the Porcine CD169 cell surface antigen, also known as Sialoadhesin, which is expressed by subsets of cells of the macrophage/monocyte cell lineage. CD169 is preferentially expressed on cells in the late stages of maturation. It is expressed strongly on alveolar and tissue macrophages, but weakly on blood monocytes.
Formulation	PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	Entrez Gene 397623 Pig UniProt ID A7LCJ3
Background	Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
Synonyms	Sialoadhesin, Siglec-1
Reference Data

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