CD172a / SIRPA Mouse Monoclonal Antibody [Clone ID: BL1H7]

Specifications

Product Data
Clone Name	BL1H7
Applications	FC, IHC, IP, WB
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment (Sodium citrate buffer pH 6.0) prior to staining of paraffin sections. Recommended Positve Control: Porcine Spleen Tissue. Western Blot: Clone BL1H7 recognizes Porcine CD172a under non-reducing conditions. Detects a band of approximately 90-115 kDa in alveolar macrophage lysates. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/10-1/20 diluted antibody to label 1x106 cells in 100 µl.
Reactivity	Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Porcine alveolar macrophages.
Specificity	This antibody is specific for the porcine CD172a antigen, also known as SWC3, which is a member of the signal regulatory protein (SIRP) family. CD172a is expressed by granulocytes, monocytes and macrophages.
Formulation	PBS State: Purified State: Liquid purfied IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Database Link	UniProt ID F1S887
Background	Protein tyrosine phosphatases (PTPases) SHP1 and SHP2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1),SHPS1 , MyD1, BIT and p84. SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP2. SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Synonyms	SHP substrate 1, SHPS-1, Sirp-alpha-2, Sirp-alpha-3, MyD-1 antigen, p84, BIT, MFR, MYD1, PTPNS1, SHPS1
Reference Data

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