Nestin (NES) Mouse Monoclonal Antibody [Clone ID: 10C2]

Specifications

Product Data
Clone Name	10C2
Applications	IF, IHC, WB
Recommend Dilution	Immunoblotting (Western). Recommended Positive Control:WI38 (ATCC# CCL75) or U251 cell lysate. Immunocytochemistry on fixed cells. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections. Recommended Dilutions: 1/100-1/200 for Immunocytochemistry and Immunohistochemistry with a Fluorescein or Rhodamine conjugated anti-Mouse secondary antibody, and 1/1000-1/5000 for Western blot applications using chemoluminescent detection.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A 150-amino-acid fragment from the cloned Human Nestin, conjugated to Glutathione S-Transferase. Monoclonal antibody 10C2 was derived by fusion of P3x63Ag8.653 myeloma cells with spleen cells from a BALB/c Mouse.
Specificity	This antibody recognizes Human Nestin protein. It does not react with Mouse and Rat Nestin.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	nestin
Database Link	Entrez Gene 10763 Human UniProt ID P48681
Background	Nestin is a large intermediate filament protein originally identified by Hockfield and McKay (1985). It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
Synonyms	NES, Nbla00170
Reference Data

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