IRF3 (108-166) Mouse Monoclonal Antibody [Clone ID: 3F10]

Specifications

Product Data
Clone Name	3F10
Applications	ELISA, FC, IF, WB
Recommend Dilution	ELISA. Western blot (1:1000 - 1:2000), recommended starting dilution is 1:1000. Flow Cytometry. Immunoflourescence.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human IRF3 (108-166 aa) purified from E.coli
Specificity	The antibody recognizes human interferon regulatory factor 3 (IRF3), a 55 kDa constitutively expressed member of the IRF family. Other species not tested.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interferon regulatory factor 3
Database Link	Entrez Gene 3661 Human UniProt ID Q14653
Background	Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection. IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta, along with other interferon-induced genes.
Synonyms	Interferon regulatory factor 3, IRF-3
Reference Data

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