Hexokinase 1 (HK1) Mouse Monoclonal Antibody [Clone ID: 4D7]

Specifications

Product Data
Clone Name	4D7
Applications	ELISA, IF, IHC, WB
Recommend Dilution	ELISA. Western blot (1:1,000). Immunofluorescence (1:1,000). Immunohistochemistry on Paraffin Sections (5 µg/ml): This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human Hexokinase 1 (1-917 aa) purified from E.coli
Specificity	The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.
Formulation	PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol State: Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Protein-G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	hexokinase 1
Database Link	Entrez Gene 3098 Human UniProt ID P19367
Background	Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.
Synonyms	HK1, Hexokinase type I, Brain form hexokinase
Reference Data

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